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. 1976 Jun 30;56(1):33-40.
doi: 10.1007/BF00293727.

Nucleoli and ploidy in Potorous cells (PTK2) in vitro

A D Branch, M W Berns
Free article

Nucleoli and ploidy in Potorous cells (PTK2) in vitro

A D Branch et al. Chromosoma. .
Free article

Abstract

Most cells of the male PTK2 cell line contain one nucleolus, and they are diploid. However, a proportion of cells have more than one nucleolus. Cells with two and three nucleoli were isolated and cloned into viable populations. Greater than 90% of the cells in these clonal populations maintained the abnormal nucleolar number of the originally isolated cell. Karyotype analysis of cells with two and three nucleoli demonstrated that the cells were respectively tetraploid and hexaploid. It was concluded that in PTK2 cells, nucleolarity is a good index of ploidy even if the ploidy level is abnormal. Furthermore, long term monitoring of the tetraploid cells demonstrated virtually no tendency towards reversion to the diploid condition as suggested by other studies in Potorous.

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References

    1. Bol Inst Estud Med Biol Univ Nac Auton Mex. 1963 Apr;21:15-23 - PubMed
    1. Exp Cell Res. 1968 Jun;50(3):599-615 - PubMed
    1. Proc Natl Acad Sci U S A. 1964 Jan;51:139-46 - PubMed
    1. J Mol Biol. 1972 Jan 14;63(1):75-83 - PubMed
    1. J Cell Biol. 1972 Jun;53(3):827-32 - PubMed

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