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. 1998 Sep 1;256(2):471-87.
doi: 10.1046/j.1432-1327.1998.2560471.x.

Sulfated di-, tri- and tetraantennary N-glycans in human Tamm-Horsfall glycoprotein

J J van Rooijen  1 , J P Kamerling, J F Vliegenthart
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Free article

Sulfated di-, tri- and tetraantennary N-glycans in human Tamm-Horsfall glycoprotein

J J van Rooijen et al. Eur J Biochem. .
Free article

Abstract

The primary structures of 32 sulfated di-, tri- and tetraantennary N-glycans of human Tamm-Horsfall glycoprotein (THP) have been determined. THP was isolated from the urine of one healthy male donor. The intact carbohydrate chains were released by PNGase-F and fractionated via FPLC on Resource Q, HPLC on LiChrosorb-NH2, and high-pH anion-exchange chromatography on CarboPac PA-1. Characterizations were performed using 500-MHz and 600-MHz 1H-NMR spectroscopy, in combination with sialidase treatments. The type of characterized N-glycans ranged from monosulfated to trisulfated N-glycans, whereby the sulfate groups were present as 3-O-sulfated Gal (Gal3S) and 4-O-sulfated GalNAc (GalNAc4S). A compilation of the established structures is shown below. [structure in text]

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