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. 1978 Dec;91(2):509-15.
doi: 10.1016/0003-2697(78)90537-7.

Quantitative Limulus lysate assay for endotoxin activity: aggregation of radioiodinated coagulogen monomers

R S Munford  1
Affiliations

Quantitative Limulus lysate assay for endotoxin activity: aggregation of radioiodinated coagulogen monomers

R S Munford. Anal Biochem. 1978 Dec.

Abstract

This communication describes a modification of the Limulus lysate assay which allows precise quantitation of picograms of bacterial lipopolysaccharide activity. The method measures the incorporation of 125I-labeled coagulogen monomers into the lysate clot as a function of lipopolysaccharide concentration. The method is more precise and requires less lysate than the previously described quantitative assays for endotoxin activity.

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