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. 1978 Dec;91(2):516-20.
doi: 10.1016/0003-2697(78)90538-9.

Measurement of S-adenosyl-L-methionine levels by SP Sephadex chromatography

R I Glazer  1 , A L Peale
Affiliations

Measurement of S-adenosyl-L-methionine levels by SP Sephadex chromatography

R I Glazer et al. Anal Biochem. 1978 Dec.

Abstract

Cation-exchange chromatography with sulfopropyl Sephadex was used to measure the levels of S-[methyl-3H]adenosyl-L-methionine synthesized by L1210 cells in vitro. Separation of S-[methyl-3H]adenosyl-L-methionine from [methyl-3H]methionine and other metabolites was achieved by stepwise elution with varying concentrations of HCl.

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