Morphological and morphometrical changes in dorsal root ganglion neurons innervating the regenerated lizard tail

Abstract

The variations occurring in neurons from dorsal root ganglia that provide innervation to the regenerated tail of the lizard (vicarious ganglia) are analysed. Vicarious ganglion neurons, when compared to control ganglion neurons (i.e. ganglia from the same animal that were not involved in the reinnervation process), show a size increase of the soma (cell hypertrophy) which applies to all cell types and subtypes. No statistically significant differences in the relative percentage of neurofilament-poor (type D) and neurofilament-rich (type L) neurons were found between vicarious dorsal root ganglia compared to controls in all animals. On the contrary, within L neuron sub-types, a statistically significant increase in sub-type L2 (very rich in neurofilaments), and the appearance of sub-type L3 neuron which is not detectable in controls, were demonstrated in vicarious dorsal root ganglia. In spite of these variations in size and percentage distribution, no structural and ultrastructural differences of the various cell types and sub-types are detectable, except for the appearance of the sub-type L3 neurons. However, this neuron sub-type might not be considered specific of hypertrophy since the same morphological features have been observed, in normal conditions, in lizard dorsal root ganglia from cervical and lumbar spinal levels that provide innervation to limb plexuses.