doi: 10.1016/0014-2999(76)90147-3.
A laminar flow superfusion technique with much increased sensitivity for the detection of smooth muscle-stimulating substances
- PMID: 976327
- DOI: 10.1016/0014-2999(76)90147-3
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A laminar flow superfusion technique with much increased sensitivity for the detection of smooth muscle-stimulating substances
Eur J Pharmacol.
1976 Oct.
Abstract
The classical superfusion technique was modified by immersing the isolated assay tissues in mineral oil, making it possible to superfuse the preparations through the oil with a drip of Krebs solution at very low flow rates (0.1 - 0.2 ml/min). In this way the sensitivity of the preparation for the detection and quantitation of endogenous biologically active substances was extended to the picogram level.
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