Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Aug;89(8):821-4.
doi: 10.1111/j.1349-7006.1998.tb00634.x.

Mutation analysis of the WT1 gene in myelodysplastic syndromes

N Hosoya  1 , K Miyagawa, K Mitani, Y Yazaki, H Hirai
Affiliations

Mutation analysis of the WT1 gene in myelodysplastic syndromes

N Hosoya et al. Jpn J Cancer Res. 1998 Aug.

Abstract

The WT1 tumor suppressor gene was examined for mutations in a panel of 44 patients with myelodysplastic syndromes (MDS) including acute myelogenous leukemias (AML) secondary to MDS, using polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis and sequencing analysis. A WT1 mutation was detected in one out of 17 cases of AML secondary to MDS. This mutation exists upstream of the zinc finger region and is predicted to produce a truncated WT1 protein lacking the zinc finger region. No mutations were detected in 27 MDS patients who had not progressed to AML. This is the first report of analysis for WT1 mutations in a large number of MDS patients, suggesting that WT1 mutations are uncommon in MDS. Abnormalities in this gene may, however, contribute to a small proportion of cases showing progression from MDS into AML.

PubMed Disclaimer

References

    1. ) Call , K. M. , Glaser , T. , Ito , C. Y. , Buckler , A. J. , Pelletier , J. , Haber , D. A. , Rose , E. A. , Kral , A. , Yeger , H. , Lewis , W. H. , Jones , C. and Housman , D. E.Isolation and characterization of a zinc finger polypeptide gene at the human chromosome 11 Wilms' tumor locus . Cell , 60 , 509 – 520 ( 1990. ). - PubMed
    1. ) Gessler , M. , Poustka , A. , Cavenee , W. , Neve , R. L. , Orkin , S. H. and Bruns , G. A.Homozygous deletion in Wilms tumours of a zinc‐finger gene identified by chromosome jumping . Nature , 343 , 774 – 778 ( 1990. ). - PubMed
    1. ) Haber , D. A. , Sohn , R. L. , Buckler , A. J. , Pelletier , J. , Call , K. M. and Housman , D. E.Alternative splicing and genomic structure of the Wilms' tumor gene WT1 . Proc. Natl. Acad. Sci. USA , 88 , 9618 – 9622 ( 1991. ). - PMC - PubMed
    1. ) Rauscher , F. J. , IIIThe WT1 Wilms tumor gene product: a developmentally regulated transcription factor in the kidney that functions as a tumor suppressor . FASEB J. , 7 , 896 – 903 ( 1993. ). - PubMed
    1. ) Miyagawa , K. , Kent , J. , Moore , A. , Charlieu , J. P. , Little , M. H. , Williamson , K. A. , Kelsey , A. , Brown , K. W. , Hassam , S. , Briner , J. , Hayashi , Y. , Hirai , H. , Yazaki , Y. , van Heyningen , V. and Hastie , N. D.Loss of WT1 function leads to ectopic myogenesis in Wilms' tumour . Nat. Genet. , 18 , 15 – 17 ( 1998. ). - PubMed

MeSH terms