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Comparative Study
. 1998 Aug;89(8):849-54.
doi: 10.1111/j.1349-7006.1998.tb00639.x.

Sequence analysis of genes encoding rodent homologues of the human tumor-rejection antigen SART-1

M Gotoh  1 , S Shichijo, T Hoshino, Y Imai, T Imaizumi, Y Inoue, H Takasu, T Yamaoka, K Itoh
Affiliations
Comparative Study

Sequence analysis of genes encoding rodent homologues of the human tumor-rejection antigen SART-1

M Gotoh et al. Jpn J Cancer Res. 1998 Aug.

Abstract

Human SART-1 (hSART-1) gene encodes a 125 kD protein with a leucine-zipper motif expressed in the nucleus of all proliferating cells, and a 43 kD protein expressed in the cytosol of most epithelial cancers. In this study, two rodent genes (rSART-1 and mSART-1) homologous to hSART-1 were cloned from cDNA libraries of murine brain and a rat tumor cell line, respectively. mSART-1 and rSART-1 were highly homologous to hSART-1 with 86% and 84% identity at the nucleotide level, and 95% and 91% at the protein level, respectively. The leucine zipper domain and two basic amino acid portions that bind DNA, as well as peptide sequences recognized by human cytotoxic T lymphocytes (CTLs), were all conserved in these rodent genes. Nuclear protein homologous to the 125 kD hSART-1(800) protein, but not to the 43 kD cytosol SART-1(259) protein, was detectable with specific antibody in the nuclear fractions of rodent tumor cell lines, and normal rodent fetal liver and testis. These rodent genes should be a novel tool for studies on the biological roles of the SART-1 gene, and also in the construction of animal models of specific immunotherapy using SART-1 gene products.

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