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. 1976 Aug;357(8):1139-43.
doi: 10.1515/bchm2.1976.357.2.1139.

Protection of actin from heat denaturation by various phallotoxins

J X de Vries, A J Schäfer, H Faulstich, T Wieland

Protection of actin from heat denaturation by various phallotoxins

J X de Vries et al. Hoppe Seylers Z Physiol Chem. 1976 Aug.

Abstract

Phallotoxins protect F-actin from heat denaturation at a temperature of 70 degrees C for 3 min. This has been shown by difference spectroscopy. G-actin is not protected from heat denaturation by phalloidin. Among the various phallopeptides investigated, 4 toxic ones showed the same protecting ability, whereas non-toxic seco-compounds has no effect. The non-toxic (S)-phalloidinsulfoxide A exhibited only a partial protecting activity, corresponding to an affinity for F-actin of about one tenth of that of the toxic peptides. Evidence for this was obtained from the easy displacement of the sulfoxide A from F-actin by phalloidin as well as by a spectroscopic dilution titration.

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