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. 1998 Jul;43(1):22-8.
doi: 10.1136/gut.43.1.22.

Effect of sodium ion coupled nutrient transport on intestinal permeability in chronically catheterised rats

M R Uhing  1
Affiliations

Effect of sodium ion coupled nutrient transport on intestinal permeability in chronically catheterised rats

M R Uhing. Gut. 1998 Jul.

Abstract

Background: The significance of Nanutrient cotransport induced alterations in paracellular permeability is controversial. Most previous studies have measured intestinal permeability using in vitro methods or in vivo methods immediately after surgical bowel manipulation, and therefore may not be applicable to normal physiological conditions.

Aims: To determine whether activation of Na coupled nutrient transport increases intestinal permeability under normal physiological conditions.

Methods: The effect of Na-nutrient cotransport on intestinal permeability was measured in unrestrained, unanaesthetised, chronically catheterised male Sprague-Dawley rats using two different methods: measurement of the rate of absorption of passively absorbed hexoses, mannitol and L-glucose; and measurement of the mannitol:urea diffusion ratio.

Results: L-Glucose and mannitol absorption were not increased in the presence of D-glucose, alanine, maltose, or peptides. The mannitol:urea diffusion ratio was not increased by the presence of D-glucose. The presence of D-glucose in the intestinal lumen for 20 minutes did not alter intestinal permeability.

Conclusions: Under normal physiological conditions, Na coupled nutrient transport does not increase intestinal permeability.

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Figures

Figure 1
Figure 1
Effect of Na coupled transport on the absorption rate of L-glucose. Bars represent mean (SD); n=9.
Figure 2
Figure 2
Effect of alanine and peptides on the rate of L-glucose absorption. Bars represent mean (SD); n=6.
Figure 3
Figure 3
Effect of Na coupled transport on the absorption rate of mannitol. Bars represent mean (SD); n=9.
Figure 4
Figure 4
Effect of infusing luminal glucose 10 minutes prior to measuring the rate of mannitol absorption at a concentration of 100 mM. Group A, n=8; group B, n=8. Bars represent mean (SD).
Figure 5
Figure 5
The effect of D-glucose on the diffusion ratio (DR) of mannitol:urea. Bars represent mean (SD).
Figure 6
Figure 6
Effect of D-glucose on the diffusion ratio (DR) of mannitol:THO (n=6).
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References

    1. J Clin Invest. 1967 Mar;46(3):287-98 - PubMed
    1. J Gen Physiol. 1954 Nov 20;38(2):225-43 - PubMed
    1. Naunyn Schmiedebergs Arch Pharmacol. 1973;279(2):133-52 - PubMed
    1. J Supramol Struct. 1977;7(1):1-13 - PubMed
    1. J Cell Biol. 1983 Jul;97(1):125-36 - PubMed