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. 1998 Nov;36(11):3178-81.
doi: 10.1128/JCM.36.11.3178-3181.1998.

Enzyme-linked immunosorbent assay based on recombinant human group C rotavirus inner capsid protein (VP6) To detect human group C rotaviruses in fecal samples

V L James  1 , P R Lambden, E O Caul, I N Clarke
Affiliations

Enzyme-linked immunosorbent assay based on recombinant human group C rotavirus inner capsid protein (VP6) To detect human group C rotaviruses in fecal samples

V L James et al. J Clin Microbiol. 1998 Nov.

Abstract

A recent study showed that 43% of a population in the United Kingdom were seropositive for group C rotavirus. The higher than expected incidence may be due to limited diagnosis of acute human group C rotavirus infections because no routine test is available. Human group C rotavirus infections are routinely diagnosed by electron microscopy (EM) and a negative group A rotavirus enzyme-linked immunosorbent assay (ELISA) result. An antigen-detection ELISA was developed with hyperimmune antibodies raised to human group C rotavirus recombinant VP6 (Bristol strain) expressed in insect cells. The assay was used to screen fecal samples to determine the prevalence of group C rotavirus infection. Samples positive by ELISA were confirmed by EM, polyacrylamide gel electrophoresis of double-stranded RNA, or detection of the VP6 gene by reverse transcription-PCR. Retrospective analysis indicated a 1 to 2% detection rate of positivity among samples from patients with acute diarrhea.

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Figures

FIG. 1
FIG. 1
ELISA of fecal samples containing identified viruses. Plot of the A450 with hyperimmune rabbit antisera against the P:N ratios for fecal samples containing various viruses identified by EM. The cutoff was derived from the mean values for the non-group C rotavirus-containing fecal samples +3 SDs, as indicated. ⧫, non-group C rotavirus-containing fecal samples; ■, samples confirmed to contain human group C rotavirus.
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