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. 1998 Nov;36(11):3355-8.
doi: 10.1128/JCM.36.11.3355-3358.1998.

Detection and typing of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks attached to human skin by PCR

G Liebisch  1 , B Sohns, W Bautsch
Affiliations

Detection and typing of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks attached to human skin by PCR

G Liebisch et al. J Clin Microbiol. 1998 Nov.

Abstract

Live Ixodes ricinus ticks attached to humans residing in Germany were examined for borreliae by dark-field microscopy and PCR. Borrelia species were identified by 16S rRNA sequence analysis, which showed the presence of several species, some not yet defined, and a high prevalence of multiply infected ticks.

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Figures

FIG. 1
FIG. 1
Alignment of the 16S rRNA sequences of B. burgdorferi: sensu stricto (BB), B. afzelii (BA), B. garinii (BG), strain VS116, and sample M94496. Only parts of the 16S rRNA sequences (from position 39 to position 735) are shown. Variant base positions are indicated by an asterisk and boldface type; the positions of the genus-specific (P1 and P2) and VS116-specific (P8 and P9) primer pairs are underlined.
FIG. 2
FIG. 2
Typing results of the PCR-positive tick homogenates. (A) Number of homogenates harboring the different genospecies; (B) combined analysis of the PCR typing results. B. burgd. s.s., B. burgdorferi sensu stricto; genus spec., genus specific; Ba, B. afzelii; Bg, B. garinii; Bb, B. burgdorferi sensu stricto.
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