Comparison of four serological tests to determine the CagA or VacA status of Helicobacter pylori strains

doi:10.1128/JCM.36.11.3433-3434.1998

Comparative Study

. 1998 Nov;36(11):3433-4.

doi: 10.1128/JCM.36.11.3433-3434.1998.

Comparison of four serological tests to determine the CagA or VacA status of Helicobacter pylori strains

Y Yamaoka¹, T Kodama, D Y Graham, K Kashima

Affiliations

PMID: 9774616
PMCID: PMC105352
DOI: 10.1128/JCM.36.11.3433-3434.1998

Comparative Study

Comparison of four serological tests to determine the CagA or VacA status of Helicobacter pylori strains

Y Yamaoka et al. J Clin Microbiol. 1998 Nov.

. 1998 Nov;36(11):3433-4.

doi: 10.1128/JCM.36.11.3433-3434.1998.

Authors

Y Yamaoka¹, T Kodama, D Y Graham, K Kashima

Affiliation

¹ Department of Medicine, Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas 77030, USA. yoshio@wt.net

PMID: 9774616
PMCID: PMC105352
DOI: 10.1128/JCM.36.11.3433-3434.1998

Abstract

We compared four tests for antibodies to CagA or VacA, HelicoBlot 2. 0, RIDA Blot Helicobacter, CHIRON RIBA H. pylori SIA, and an enzyme-linked immunosorbent assay using recombinant CagA. Immunoblot assays were accurate for determining Helicobacter pylori status but poor for determining CagA or VacA status (accuracy, 66 to 80% for CagA status and 34 to 67% for VacA status). None can be recommended for determining CagA or VacA status.

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References

1. Blaser M J, Perez-Perez G I, Kleanthous H, Cover T L, Peek R M, Chyou P H, Stemmermann G N, Nomura A. Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Res. 1995;55:2111–2115. - PubMed
1. Ching C K, Wong B C Y, Kwok E, Ong L, Covacci A, Lam S K. Prevalence of CagA-bearing Helicobacter pylori strains detected by the anti-CagA assay in patients with peptic ulcer disease and in controls. Am J Gastroenterol. 1996;91:949–953. - PubMed
1. Cover T L, Dooley C P, Blaser M J. Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolating cytotoxin activity. Infect Immun. 1990;58:603–610. - PMC - PubMed
1. Cover T L, Glupczynski Y, Lage A P, Burette A, Tummuru M K R, Perez-Perez G I, Blaser M J. Serologic detection of infection with cagA+Helicobacter pylori strains. J Clin Microbiol. 1995;33:1496–1500. - PMC - PubMed
1. Graham D Y, Genta R M, Graham D P, Crabtree J E. Serum CagA antibodies in asymptomatic subjects and patients with peptic ulcer: lack of correlation of IgG antibody in patients with peptic ulcer or asymptomatic Helicobacter pylori gastritis. J Clin Pathol. 1996;49:829–832. - PMC - PubMed

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[1] Blaser M J, Perez-Perez G I, Kleanthous H, Cover T L, Peek R M, Chyou P H, Stemmermann G N, Nomura A. Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Res. 1995;55:2111–2115. - PubMed

[2] Blaser M J, Perez-Perez G I, Kleanthous H, Cover T L, Peek R M, Chyou P H, Stemmermann G N, Nomura A. Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Res. 1995;55:2111–2115. - PubMed

[3] Ching C K, Wong B C Y, Kwok E, Ong L, Covacci A, Lam S K. Prevalence of CagA-bearing Helicobacter pylori strains detected by the anti-CagA assay in patients with peptic ulcer disease and in controls. Am J Gastroenterol. 1996;91:949–953. - PubMed

[4] Ching C K, Wong B C Y, Kwok E, Ong L, Covacci A, Lam S K. Prevalence of CagA-bearing Helicobacter pylori strains detected by the anti-CagA assay in patients with peptic ulcer disease and in controls. Am J Gastroenterol. 1996;91:949–953. - PubMed

[5] Cover T L, Dooley C P, Blaser M J. Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolating cytotoxin activity. Infect Immun. 1990;58:603–610. - PMC - PubMed

[6] Cover T L, Dooley C P, Blaser M J. Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolating cytotoxin activity. Infect Immun. 1990;58:603–610. - PMC - PubMed

[7] Cover T L, Glupczynski Y, Lage A P, Burette A, Tummuru M K R, Perez-Perez G I, Blaser M J. Serologic detection of infection with cagA+Helicobacter pylori strains. J Clin Microbiol. 1995;33:1496–1500. - PMC - PubMed

[8] Cover T L, Glupczynski Y, Lage A P, Burette A, Tummuru M K R, Perez-Perez G I, Blaser M J. Serologic detection of infection with cagA+Helicobacter pylori strains. J Clin Microbiol. 1995;33:1496–1500. - PMC - PubMed

[9] Graham D Y, Genta R M, Graham D P, Crabtree J E. Serum CagA antibodies in asymptomatic subjects and patients with peptic ulcer: lack of correlation of IgG antibody in patients with peptic ulcer or asymptomatic Helicobacter pylori gastritis. J Clin Pathol. 1996;49:829–832. - PMC - PubMed

[10] Graham D Y, Genta R M, Graham D P, Crabtree J E. Serum CagA antibodies in asymptomatic subjects and patients with peptic ulcer: lack of correlation of IgG antibody in patients with peptic ulcer or asymptomatic Helicobacter pylori gastritis. J Clin Pathol. 1996;49:829–832. - PMC - PubMed

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Comparison of four serological tests to determine the CagA or VacA status of Helicobacter pylori strains

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Comparison of four serological tests to determine the CagA or VacA status of Helicobacter pylori strains

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