Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Nov 1;26(21):5013-5.
doi: 10.1093/nar/26.21.5013.

Primer specific and mispair extension analysis (PSMEA) as a simple approach to fast genotyping

Y W Hu  1 , E Balaskas, G Kessler, C Issid, L J Scully, D G Murphy, A Rinfret, A Giulivi, V Scalia, P Gill
Affiliations

Primer specific and mispair extension analysis (PSMEA) as a simple approach to fast genotyping

Y W Hu et al. Nucleic Acids Res. .

Abstract

A simple method, primer specific and mispair extension analysis (PSMEA) with pfu DNA polymerase was developed for genotyping. PSMEA is based on the unique properties of 3'-->5' exonuclease proofreading activity. In the presence of an incomplete set of dNTPs, pfu was found to be extremely discriminative in nucleotide incorporation and proofreading at the initiation step of DNA synthesis, completely preventing primer extension when mispair(s) are found adjacent to the 3'-end of the primer. This has allowed us to accurately detect nucleotide variations, deletions and insertions for fast genotyping.

PubMed Disclaimer