Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Nov 3:126:679-91.
doi: 10.1016/s0021-9673(01)84111-x.

Evaluation of microparticle chemically bonded reversed-phase packings in the high-pressure liquid chromatographic analysis of nucleosides and their bases

R A Hartwick, P R Brown

Evaluation of microparticle chemically bonded reversed-phase packings in the high-pressure liquid chromatographic analysis of nucleosides and their bases

R A Hartwick et al. J Chromatogr. .

Abstract

The reversed-phase partition mode of high-pressure liquid chromatography was used for the analysis of seven of the naturally occurring nucleosides and their bases. With microparticle chemically bonded packings, nucleosides and their bases can be quantitatively determined in the presence of nucleotides in 30 min with high sensitivity, accuracy, and reproducibility. Peaks in chromatograms of cell extracts were identified by absorbance ratios and enzymatic peak shift methods. Applications of this technique to biochemical studies are reported.

PubMed Disclaimer

Publication types