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Comparative Study
. 1998 Oct;74(2):209-13.
doi: 10.1016/s0166-0934(98)00049-4.

Hepatitis E virus RNA detection in serum and feces specimens with the use of microspin columns

R Aggarwal  1 , K A McCaustland
Affiliations
Comparative Study

Hepatitis E virus RNA detection in serum and feces specimens with the use of microspin columns

R Aggarwal et al. J Virol Methods. 1998 Oct.

Abstract

This report describes the use of microspin columns for extraction of hepatitis E virus (HEV) RNA from stool and serum specimens for reverse transcription-polymerase chain reaction (RT-PCR) and compares this method with the glass powder method. The microspin column method was found to be 1- to 2-log more sensitive in detecting HEV RNA than the glass powder method and had better reproducibility. The microspin column method also detected HEV RNA in a larger number of specimens than the glass powder method from among a panel of serum and stool specimens. Use of this method may allow better assessment of viremia and fecal excretion in patients and experimental animals infected with HEV.

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