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. 1998;4(5):303-10.
doi: 10.1002/(SICI)1520-6343(1998)4:5%3C303::AID-BSPY2%3E3.0.CO;2-X.

Spatially localized ballistic two-photon excitation in scattering media

H Szmacinski  1 , I Gryczynski, J R Lakowicz
Affiliations

Spatially localized ballistic two-photon excitation in scattering media

H Szmacinski et al. Biospectroscopy. 1998.

Abstract

We describe spatially localized two-photon excitation in scattering media. Using femtosecond pulses at 770 nm from a Ti: Sapphire laser, we were able to excite fluorophores in capillary tubes under up to 1.5 mm of 0.5% intralipid. Displacement of the laser beam relative to the embedded samples indicates that highly localized excitation was possible with two-photon excitation, whereas one-photon excitation resulted in loss of spatial resolution due to excitation by the diffusely scattered photons. These results indicate that two-photon excitation in the scattering solution is due only to the ballistic photons, a result confirmed by frequency-domain time-resolved measurements. Selective excitation of adjacent embedded samples was found possible for two but not one-photon excitation.

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