Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 1998 Oct;10(2):155-65.
doi: 10.1007/BF02760862.

Differential display of mRNA

J S Zhang  1 , E L Duncan, A C Chang, R R Reddel
Affiliations
Review

Differential display of mRNA

J S Zhang et al. Mol Biotechnol. 1998 Oct.

Abstract

Differential display of mRNA (DD) is a technique in which mRNA species expressed by a cell population are reverse transcribed and then amplified by many separate polymerase chain reactions (PCR). PCR primers and conditions are chosen so that any given reaction yields a limited number of amplified cDNA fragments, permitting their visualization as discrete bands following gel electrophoresis. This robust and relatively simple procedure allows identification of genes that are differentially expressed in different cell populations. Here we review DD including some recent modifications, and compare it with other techniques for analyzing differential mRNA expression.

PubMed Disclaimer

References

    1. Biotechniques. 1995 Feb;18(2):200-2 - PubMed
    1. Trends Genet. 1996 Oct;12(10):396-7 - PubMed
    1. Nucleic Acids Res. 1994 Dec 25;22(25):5763-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3275-9 - PubMed
    1. Methods Mol Biol. 1997;85:3-11 - PubMed

MeSH terms

LinkOut - more resources