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. 1998 Oct;14(4):121-4.
doi: 10.1016/s1050-3862(98)00009-6.

Estimation of DNA single-strand breaks by single cells gel electrophoresis in tumor cells

S Neubauer  1 , T Liehr, S Birkenhake, E Gebhart, R Fietkau, R Sauer
Affiliations

Estimation of DNA single-strand breaks by single cells gel electrophoresis in tumor cells

S Neubauer et al. Genet Anal. 1998 Oct.

Abstract

To estimate the frequency of single-strand breaks on a single cell level the so-called comet-assay (single cell gel electrophoresis) is a well-established technique. We present a modified protocol suitable for testing primary tumors, a kind of tissue very uneasy to be analysed in former single cell gel electrophoresis assays. Tumor cells of 12 studied cases showed a typical dose-rate effect on in vitro irradiation with different X-ray doses, as observed in peripheral blood leukocytes. Interestingly, the repair capability of primary tumor cells was lower than that of peripheral blood leukocytes of the same patients.

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