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. 1998 Dec;36(12):1843-50.
doi: 10.1016/s0041-0101(98)00105-6.

Cloning of cDNAS encoding neurotoxic peptides from the spider Phoneutria nigriventer

E Kalapothakis  1 , C L Penaforte, P S Beirão, M A Romano-Silva, J S Cruz, M A Prado, P E Guimarães, M V Gomez, V F Prado
Affiliations

Cloning of cDNAS encoding neurotoxic peptides from the spider Phoneutria nigriventer

E Kalapothakis et al. Toxicon. 1998 Dec.

Abstract

A cDNA library made from venom glands of the spider Phoneutria nigriventer was constructed and used to clone neurotoxic peptides. A cDNA of about 360 nucleotides encoding the precursor for the toxin Tx2-1 active on mammals has been isolated. The deduced amino acid sequence for the mature polypeptide confirms the polypeptide sequence previously published. In addition, two new putative toxins called Pn2-1A and Pn2-5A have been characterized and their complete amino acid sequence show 92% similarity to Tx2-1 and 94% similarity to Tx2-5 respectively. The cDNAs revealed that the precursors contain signal peptides characterized by a very hydrophobic core and a propeptide interposed between the signal sequence and the peptide toxin.

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