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Comparative Study
. 1998 Nov;14(15):1399-406.
doi: 10.1002/(SICI)1097-0061(199811)14:15<1399::AID-YEA324>3.0.CO;2-D.

Isolation and sequence analysis of the orotidine-5'-phosphate decarboxylase gene (URA3) of Candida utilis. Comparison with the OMP decarboxylase gene family

L Rodríguez  1 , F P Chávez, M E González, L Basabe, T Rivero
Affiliations
Comparative Study

Isolation and sequence analysis of the orotidine-5'-phosphate decarboxylase gene (URA3) of Candida utilis. Comparison with the OMP decarboxylase gene family

L Rodríguez et al. Yeast. 1998 Nov.

Abstract

The URA3 gene of Candida utilis encoding orotidine-5'-phosphate decarboxylase enzyme was isolated by complementation in Escherichia coli pyrF mutation. The deduced amino-acid sequence is highly similar to that of the Ura3 proteins from other yeast and fungal species. An extensive analysis of the family of orotidine-5'-phosphate decarboxylase is shown. The URA3 gene of C. utilis was able to complement functionally the ura3 mutation of Saccharomyces cerevisiae.

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