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Comparative Study
. 1976 Aug;14(7-8):547-60.
doi: 10.1007/BF00485834.

Characterization of ADP-glucose pyrophosphorylase from shrunken-2 and brittle-2 mutants of maize

L C Hannah, O E Nelson Jr
Comparative Study

Characterization of ADP-glucose pyrophosphorylase from shrunken-2 and brittle-2 mutants of maize

L C Hannah et al. Biochem Genet. 1976 Aug.

Abstract

Electrophoretic characterization of adenosine diphosphate glucose pyrophosphorylase from the developing endosperms of nine shrunken-2 and four brittle-2 mutants revealed that (1) all mutants had low but detectable levels of activity, (2) mutation at either locus decreased activity of pyrophosphorylases A and B, and (3) differences in mobility were not found. However, pyrophosphorylase B extracted from several shrunken-2 and brittle-2 mutants differed from normal in extent of urea denaturation, Km (glucose-1-phosphate) or type of glucose-1-phosphaociation with the sh2 locus) appears to differ from normal in Km (glucose-1-phosphate).

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References

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