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. 1999 Jan;37(1):221-4.
doi: 10.1128/JCM.37.1.221-224.1999.

Dissemination of high-level penicillin-, extended-spectrum cephalosporin-, and erythromycin-resistant Streptococcus pneumoniae clones in Taiwan

P R Hsueh  1 , L J Teng, L N Lee, P C Yang, S W Ho, K T Luh
Affiliations

Dissemination of high-level penicillin-, extended-spectrum cephalosporin-, and erythromycin-resistant Streptococcus pneumoniae clones in Taiwan

P R Hsueh et al. J Clin Microbiol. 1999 Jan.

Abstract

Sixty-seven clinical isolates of Streptococcus pneumoniae (40 of serotype 23F, 19 of serotype 19F, and 8 of serotype 6B) with decreased susceptibilities to penicillin and erythromycin were characterized by antimicrobial susceptibility patterns; DNA restriction endonuclease cleavage profiles of the penicillin-binding protein genes pbp1a, pbp2b, and pbp2x; random amplified polymorphic DNA (RAPD) patterns generated by arbitrarily primed PCR; and chromosomal macrorestriction profiles based on pulsed-field gel electrophoresis. A total of 22 clones (identical or closely related pulsotypes and identical RAPD patterns) were identified; 14 clones of 23F, 6 of 19F, and 2 of 6B. Three 23F clones (26 isolates) and one 19F clone (9 isolates) expressed high-level resistance to penicillin, cefotaxime, and erythromycin (MICs >/= 256 microg/ml). These data strongly suggest that multiple high-level penicillin-, extended-spectrum cephalosporin-, and macrolide-resistant clones of S. pneumoniae have been disseminated in Taiwan.

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Figures

FIG. 1
FIG. 1
RAPD patterns generated by arbitrarily primed PCR of the 22 clones of S. pneumoniae with two primers, M13 and ERIC1. Molecular sizes (lane M) are indicated in kilobases (Gibco BRL).
FIG. 2
FIG. 2
PFGE profiles of SmaI-digested chromosomal DNA of nine clones of serotype 23F S. pneumoniae isolates. Lane M is a molecular size standard for a bacteriophage λ ladder (Gibco BRL); lanes 1 to 9 show clones 1 to 9, respectively.
See this image and copyright information in PMC

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