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. 1998 Sep;43(3):428-32.
doi: 10.1136/gut.43.3.428.

Enhanced expression of monocyte tissue factor in patients with liver cirrhosis

M Saliola  1 , R Lorenzet, D Ferro, S Basili, C Caroselli, A D Santo, M Sallese, F Violi
Affiliations

Enhanced expression of monocyte tissue factor in patients with liver cirrhosis

M Saliola et al. Gut. 1998 Sep.

Abstract

Background: Previous studies have shown that cirrhotic patients produce increased amounts of thrombin but the underlying mechanism is still unknown.

Aims: To analyse the relation between the rate of thrombin generation and monocyte expression of tissue factor (TF) in cirrhosis.

Patients: Thirty three cirrhotic patients classified as having low (n = 7), moderate (n = 17), or severe (n = 9) liver failure according to Child-Pugh criteria.

Methods: Prothrombin fragment F1 + 2, monocyte TF activity and antigen, and endotoxaemia were measured in all patients. Polymerase chain reaction (PCR) analysis of TF mRNA was performed in monocytes of five cirrhotic patients.

Results: Prothrombin fragment F1 + 2 was higher in cirrhotic patients than in controls (p < 0.0001). Monocytes from cirrhotic patients had higher TF activity and antigen than those from controls (p < 0.001) with a progressive increase from low to severe liver failure. Monocyte expression of TF was significantly correlated with plasma levels of F1 + 2 (TF activity: r = 0.98, p < 0.0001; TF antigen: r = 0.95, p < 0.0001) and with endotoxaemia (TF activity: r = 0.94, p < 0.0001; TF antigen: r = 0.91, p < 0.0001). PCR analysis of TF mRNA showed TF expression only in three patients with endotoxaemia (more than 15 pg/ml).

Conclusions: Cirrhotic patients have enhanced expression of TF which could be responsible for clotting activation, suggesting that endotoxaemia might play a pivotal role.

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Figures

Figure 1
Figure 1
Tissue factor activity in unstimulated monocytes from controls and from cirrhotic patients with low (grade A), moderate (grade B), and severe (grade C) liver failure.
Figure 2
Figure 2
Tissue factor antigen in unstimulated monocytes from controls and from patients with low (grade A), moderate (grade B), and severe (grade C) liver failure.
Figure 3
Figure 3
Correlation between tissue factor activity in unstimulated monocytes and prothrombotic fragment F1+2 in cirrhotic patients.
Figure 4
Figure 4
Correlation between tissue factor antigen in unstimulated monocytes and prothrombin fragment F1+2 in cirrhotic patients.
Figure 5
Figure 5
Tissue factor mRNA levels in monocytes from cirrhotic patients. Patient numbers (1-5) correspond to those reported in table 2.
See this image and copyright information in PMC

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