Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Jun 29;364(1):83-9.
doi: 10.1007/BF01062916.

Renal test dyes IV. Intravital Fluorescence microscopy and microphotometry of the tubularly secreted dye sulfonefluorescein

M Steinhausen, P Müller, N Parekh

Renal test dyes IV. Intravital Fluorescence microscopy and microphotometry of the tubularly secreted dye sulfonefluorescein

M Steinhausen et al. Pflugers Arch. .

Abstract

The present study describes for the first time the use of the fluorescent dye sulfonefluorescein for intravital microscopy and microphotometry on the renal surface of rats. With help of this dye tubular secretion can be observed in mammalian kidney in vivo. Tubular secretion can further be quantified with microphotometrical measurements. The molecular structure of sulfonefluorescein is closely related to phenol red, which is known for its tubular secretion. Clearance experiments also show a secretion of sulfonefluorescein. The secretion can be inhibited by probenecid. Ureter ligation causes a strong increase in tubular concentration of the dye. After a temporary ischemia dye accumulation in tubular lumen is greatly reduced.

PubMed Disclaimer

References

    1. Am J Physiol. 1964 Apr;206:687-93 - PubMed
    1. Am J Physiol. 1958 Jul;194(1):135-42 - PubMed
    1. Klin Wochenschr. 1955 Aug 1;33(29-30):729-30 - PubMed
    1. Pflugers Arch. 1975 Jun 26;357(3-4):149-63 - PubMed
    1. Am J Physiol. 1966 Nov;211(5):1152-64 - PubMed