doi: 10.1016/s0960-894x(98)00177-2.
Recognition of a non-standard base pair by thermostable DNA polymerases
Affiliations
- PMID: 9871725
- DOI: 10.1016/s0960-894x(98)00177-2
Item in Clipboard
Recognition of a non-standard base pair by thermostable DNA polymerases
Bioorg Med Chem Lett.
.
Abstract
Examination of several commercially available thermostable DNA polymerases identifies 9 degrees N DNA polymerase as single enzyme that could incorporate two components of an expanded genetic alphabet, 2,4-diaminopyrimidine and xanthosine as deoxynucleoside triphosphate opposite their cognate base in a DNA template.
Similar articles
-
Differential discrimination of DNA polymerase for variants of the non-standard nucleobase pair between xanthosine and 2,4-diaminopyrimidine, two components of an expanded genetic alphabet.Nucleic Acids Res. 1996 Apr 1;24(7):1308-13. doi: 10.1093/nar/24.7.1308. Nucleic Acids Res. 1996. PMID: 8614635 Free PMC article.
-
Thermostable DNA polymerases can perform translesion synthesis using 8-oxoguanine and tetrahydrofuran-containing DNA templates.Biochim Biophys Acta. 2006 Jan;1764(1):97-104. doi: 10.1016/j.bbapap.2005.11.001. Epub 2005 Dec 1. Biochim Biophys Acta. 2006. PMID: 16338185
-
In vitro replication slippage by DNA polymerases from thermophilic organisms.J Mol Biol. 2001 Sep 14;312(2):323-33. doi: 10.1006/jmbi.2001.4943. J Mol Biol. 2001. PMID: 11554789
-
Thermostable DNA polymerases.Adv Protein Chem. 1996;48:377-435. doi: 10.1016/s0065-3233(08)60367-8. Adv Protein Chem. 1996. PMID: 8791630 Review. No abstract available.
-
The mechanism of action of T7 DNA polymerase.Curr Opin Struct Biol. 1998 Dec;8(6):704-12. doi: 10.1016/s0959-440x(98)80089-4. Curr Opin Struct Biol. 1998. PMID: 9914251 Review.
Cited by
-
Unnatural base pairs for specific transcription.Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):4922-5. doi: 10.1073/pnas.091532698. Proc Natl Acad Sci U S A. 2001. PMID: 11320242 Free PMC article.
-
Mechanistic studies in the synthesis of a series of thieno-expanded xanthosine and guanosine nucleosides.Tetrahedron. 2008 Nov 24;64(48):10791-10797. doi: 10.1016/j.tet.2008.09.011. Tetrahedron. 2008. PMID: 19946353 Free PMC article.
-
In vitro selection using modified or unnatural nucleotides.Curr Protoc Nucleic Acid Chem. 2002 Feb;Chapter 9:Unit 9.6. doi: 10.1002/0471142700.nc0906s07. Curr Protoc Nucleic Acid Chem. 2002. PMID: 18428900 Review.
-
Unnatural substrate repertoire of A, B, and X family DNA polymerases.J Am Chem Soc. 2008 Nov 5;130(44):14872-82. doi: 10.1021/ja803833h. Epub 2008 Oct 11. J Am Chem Soc. 2008. PMID: 18847263 Free PMC article.
-
In vitro selection using modified or unnatural nucleotides.Curr Protoc Nucleic Acid Chem. 2014 Mar 26;56:9.6.1-33. doi: 10.1002/0471142700.nc0906s56. Curr Protoc Nucleic Acid Chem. 2014. PMID: 25606981 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
