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Comparative Study
. 1999 Jan;65(1):156-62.
doi: 10.1128/AEM.65.1.156-162.1999.

Morphological and biochemical properties of a Sphaerotilus sp. Isolated from paper mill slimes

V Pellegrin  1 , S Juretschko, M Wagner, G Cottenceau
Affiliations
Comparative Study

Morphological and biochemical properties of a Sphaerotilus sp. Isolated from paper mill slimes

V Pellegrin et al. Appl Environ Microbiol. 1999 Jan.

Abstract

Four strains of filamentous bacteria were isolated from slimes collected in different paper mill factories. Morphological and physiological characterization of the isolates indicated an affiliation with the genus Sphaerotilus. However, while the physiological properties of the isolates were almost identical, pronounced physiological differences between the isolates and Sphaerotilus natans DSM 6575(T), DSM 565, and DSM 566 with respect to their ability to metabolize complex polysaccharides, sugars, polyalcohols, or organic acids as carbon sources were detected. In contrast to the analyzed culture collection strains of S. natans, all paper mill isolates were able to grow at elevated temperatures of up to 40 degrees C. Comparative sequence analysis of nearly complete 16S ribosomal DNA (rDNA) sequences from the four new isolates demonstrated that the retrieved sequences were highly similar to each other (99.6 to 99.8% similarity) and to previously published partial 16S rDNA sequences of S. natans DSM 6575(T) and ATCC 15291. Polyphasic characterization of the isolated Sphaerotilus strains revealed interesting adaptations of the strains to the environmental paper mill conditions with regard to temperature tolerance and utilization of cellulose and starch.

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Figures

FIG. 1
FIG. 1
S. natans IF4 producing rough colonies on FIL agar medium (magnification, ×1.5).
FIG. 2
FIG. 2
S. natans IF14 developing rough-smooth colonies on FIL agar medium (magnification, ×2).
FIG. 3
FIG. 3
S. natans IF4 producing long unbranched and ensheathed filaments (magnification, ×284).
FIG. 4
FIG. 4
Scanning electron micrograph showing slimy matrix covering the sheath of strain IF4 (magnification, ×3,154).
FIG. 5
FIG. 5
Phylogenetic tree reflecting the relationships of the Sphaerotilus sp. isolates obtained from paper mill slime within the beta subclass of Proteobacteria. The tree is based on the results of maximum likelihood analysis. The multifurcation connects branches for which a relative order could not be unambiguously determined. The bar indicates 10% estimated sequence divergence.
See this image and copyright information in PMC

References

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