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Comparative Study
. 1999 Jan;6(1):1-5.
doi: 10.1128/CDLI.6.1.1-5.1999.

Differentiation between Mycobacterium bovis BCG-vaccinated and M. bovis-infected cattle by using recombinant mycobacterial antigens

B M Buddle  1 , N A Parlane, D L Keen, F E Aldwell, J M Pollock, K Lightbody, P Andersen
Affiliations
Comparative Study

Differentiation between Mycobacterium bovis BCG-vaccinated and M. bovis-infected cattle by using recombinant mycobacterial antigens

B M Buddle et al. Clin Diagn Lab Immunol. 1999 Jan.

Abstract

Tuberculosis continues to be a worldwide problem for both humans and animals. The development of tests to differentiate between infection with Mycobacterium tuberculosis or Mycobacterium bovis and vaccination with M. bovis BCG could greatly assist in the diagnosis of early infection as well as enhance the use of tuberculosis vaccines on a wider scale. Recombinant forms of four major secreted proteins of M. bovis-MPB59, MPB64, MPB70, and ESAT-6-were tested in a whole-blood gamma interferon (IFN-gamma) assay for differentiation between cattle vaccinated with BCG and those experimentally infected with M. bovis. BCG vaccination induced minimal protection in the present study, with similar numbers of animals infected with M. bovis in BCG-vaccinated and nonvaccinated groups. Following vaccination with BCG, the animals produced moderate IFN-gamma responses to bovine purified protein derivative (PPDB) but very weak responses to the recombinant antigens. Cattle from both the BCG-vaccinated and nonvaccinated groups which were M. bovis culture positive following challenge produced IFN-gamma responses to PPDB and ESAT-6 which were significantly stronger than those observed in the corresponding M. bovis culture-negative animals. IFN-gamma responses to MPB59, MPB64, and MPB70 were significantly weaker, and these antigens could not discriminate between vaccinated animals which develop disease and the culture-negative animals. The results of the study indicate that of the four antigens tested in the IFN-gamma assay, only ESAT-6 would be suitable for differentiating BCG-vaccinated animals from those infected with bovine tuberculosis.

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Figures

FIG. 1
FIG. 1
Effect of BCG vaccination on the levels of IFN-γ released from cultures of whole blood of calves after stimulation with PPDB. ○, BCG-vaccinated calves; ⧫, nonvaccinated calves. Arrows indicate vaccinations with BCG. Data are means ± standard errors of the means (n = 12), in ODI units.
FIG. 2
FIG. 2
Effect of BCG vaccination on the levels of IFN-γ released from cultures of whole blood of calves after stimulation with different mycobacterial antigens. Immune responses were measured at 12 weeks after the initial BCG vaccination. ■, BCG-vaccinated calves; □, nonvaccinated calves. Data are means ± standard errors of the means (n = 12), in ODI units.
FIG. 3
FIG. 3
Effect of infection with M. bovis on levels of IFN-γ released from cultures of whole blood of nonvaccinated (a) and BCG-vaccinated (b) calves after stimulation with different mycobacterial antigens. Immune responses were measured at 16 weeks after challenge with M. bovis. □, nonchallenged calves (n = 3); ■, M. bovis-challenged calves which were culture negative (n = 4); ░⃞, M. bovis-challenged calves which were infected with M. bovis (n = 5). Data are means ± standard errors of the means, in ODI units.
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