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. 1976 Sep;3(9):2303-8.
doi: 10.1093/nar/3.9.2303.

A general method for isolation of high molecular weight DNA from eukaryotes

N Blin, D W Stafford
Free PMC article

A general method for isolation of high molecular weight DNA from eukaryotes

N Blin et al. Nucleic Acids Res. 1976 Sep.
Free PMC article

Abstract

A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Crypthecodinium cuhnii, a dinoflagellate). The DNA obtained in such a way has an average molecular weight of about 200 X 10(6) d and contains very few, if any, single strand breaks.

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