Infection of tobacco or Arabidopsis plants by CMV counteracts systemic post-transcriptional silencing of nonviral (trans)genes

Abstract

Cucumber mosaic cucumovirus (CMV) infection but not tomato black ring nepovirus infection counteracted post-transcriptional gene silencing (PTGS) of nitrate reductase (Nia) or beta-glucuronidase (uidA) transgenes in newly developing leaves of tobacco and Arabidopsis plants. PTGS did not affect meristems of noninfected silenced plants, indicating that the interfering effect of CMV is not likely to occur in the meristem. Models are proposed to explain how CMV (which has no sequence similarity to the Nia or uidA transgenes) can inhibit cellular factors involved in the RNA degradation step of PTGS and/or inhibit the systemic spread of the silencing signal to tissues emerging from the meristem.