Cyclic AMP receptor protein and TyrR are required for acid pH and anaerobic induction of hyaB and aniC in Salmonella typhimurium

Abstract

Two acid-inducible genes, aniC and aciK, that require anaerobiosis and tyrosine for expression were identified as orf326a encoding a potential amino acid/polyamine antiporter and hyaB encoding hydrogenase I, respectively. Cyclic AMP (cAMP) receptor protein, cAMP, and TyrR, regulator of aromatic amino acid metabolism, were strong positive regulators of both genes.

FIG. 1

Locations of MudJ insertions and homologies. Locations of MudJ insertions in the S. typhimurium homologs of E. coli aniC (orf326a) (A), hyaB (B), and tyrR (C). The upper diagrams in panels A and B illustrate gene organization in E. coli and the approximate site of the MudJ insertion in S. typhimurium as determined by the sequence analysis shown at the bottom of each panel.