Evaluation of a colorimetric PCR-based assay to diagnose Plasmodium falciparum malaria in travelers

doi:10.1128/JCM.37.2.339-341.1999

. 1999 Feb;37(2):339-41.

doi: 10.1128/JCM.37.2.339-341.1999.

Evaluation of a colorimetric PCR-based assay to diagnose Plasmodium falciparum malaria in travelers

K J Zhong¹, K C Kain

Affiliations

PMID: 9889214
PMCID: PMC84301
DOI: 10.1128/JCM.37.2.339-341.1999

Evaluation of a colorimetric PCR-based assay to diagnose Plasmodium falciparum malaria in travelers

K J Zhong et al. J Clin Microbiol. 1999 Feb.

. 1999 Feb;37(2):339-41.

doi: 10.1128/JCM.37.2.339-341.1999.

Authors

K J Zhong¹, K C Kain

Affiliation

¹ Tropical Disease Unit, Division of Infectious Diseases, Department of Medicine, Toronto Hospital, and the University of Toronto, Toronto, Ontario, Canada.

PMID: 9889214
PMCID: PMC84301
DOI: 10.1128/JCM.37.2.339-341.1999

Abstract

New diagnostic tests are needed to facilitate the diagnosis of Plasmodium falciparum malaria in the returned traveler. We performed a blinded evaluation of a nonisotopic colorimetric PCR-based assay (Digene SHARP Signal System) and compared the results with those obtained by microscopy and nested PCR for the detection of P. falciparum malaria in 150 febrile travelers. By using nested PCR as the reference standard, the colorimetric assay had a sensitivity of 100% and a specificity of 95.4% for the detection of P. falciparum. This PCR-based nonisotopic assay is a rapid, sensitive, and specific method for the detection of falciparum malaria in returned travelers.

PubMed Disclaimer

Cited by

Comparison of IsoCode STIX and FTA Gene Guard collection matrices as whole-blood storage and processing devices for diagnosis of malaria by PCR.
Zhong KJ, Salas CJ, Shafer R, Gubanov A, Gasser RA Jr, Magill AJ, Forney JR, Kain KC. Zhong KJ, et al. J Clin Microbiol. 2001 Mar;39(3):1195-6. doi: 10.1128/JCM.39.3.1195-1196.2001. J Clin Microbiol. 2001. PMID: 11230458 Free PMC article.
Development and evaluation of a PCR-enzyme-linked immunosorbent assay for diagnosis of human brucellosis.
Morata P, Queipo-Ortuño MI, Reguera JM, García-Ordoñez MA, Cárdenas A, Colmenero JD. Morata P, et al. J Clin Microbiol. 2003 Jan;41(1):144-8. doi: 10.1128/JCM.41.1.144-148.2003. J Clin Microbiol. 2003. PMID: 12517839 Free PMC article.
Simple, rapid, inexpensive platform for the diagnosis of malaria by loop mediated isothermal amplification (LAMP).
Surabattula R, Vejandla MP, Mallepaddi PC, Faulstich K, Polavarapu R. Surabattula R, et al. Exp Parasitol. 2013 Jul;134(3):333-40. doi: 10.1016/j.exppara.2013.03.031. Epub 2013 Apr 4. Exp Parasitol. 2013. PMID: 23562879 Free PMC article.
Diagnosis of parasitic diseases: old and new approaches.
Ndao M. Ndao M. Interdiscip Perspect Infect Dis. 2009;2009:278246. doi: 10.1155/2009/278246. Epub 2009 Dec 30. Interdiscip Perspect Infect Dis. 2009. PMID: 20069111 Free PMC article.
Detection of Plasmodium Species by High-Resolution Melt Analysis of DNA from Blood Smears Acquired in Southwestern Uganda.
Kassaza K, Operario DJ, Nyehangane D, Coffey KC, Namugosa M, Turkheimer L, Ojuka P, Orikiriza P, Mwanga-Amumpaire J, Byarugaba F, Bazira J, Guler JL, Moore CC, Boum Y 2nd. Kassaza K, et al. J Clin Microbiol. 2017 Dec 26;56(1):e01060-17. doi: 10.1128/JCM.01060-17. Print 2018 Jan. J Clin Microbiol. 2017. PMID: 29046412 Free PMC article.

See all "Cited by" articles

References

1. Brown A E, Kain K C, Pipithkul J, Webster H K. Demonstration by the polymerase chain reaction of mixed Plasmodium falciparum and P. vivax infections undetected by conventional microscopy. Trans R Soc Trop Med Hyg. 1992;86:609–612. - PubMed
1. Casey J, Davidson N. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. Nucleic Acids Res. 1977;4:1539–1552. - PMC - PubMed
1. Humar A, Harrington M A, Kain K C. Evaluation of a non-isotopic polymerase chain reaction-based assay to detect and predict treatment failure of Plasmodium vivax malaria in travelers. Trans R Soc Trop Med Hyg. 1997;91:406–409. - PubMed
1. Humar A, Ohrt C, Harrington M A, Pillai D, Kain K C. ParaSight®F test compared with the polymerase chain reaction and microscopy for the diagnosis of Plasmodium falciparum malaria in travelers. Am J Trop Med Hyg. 1997;55:44–48. - PubMed
1. Kain K C, Brown A E, Mirabelli L, Webster H K. Detection of Plasmodium vivax by polymerase chain reaction in a field study. J Infect Dis. 1993;168:1323–1326. - PubMed

Publication types

Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

LinkOut - more resources

Full Text Sources

[1] Brown A E, Kain K C, Pipithkul J, Webster H K. Demonstration by the polymerase chain reaction of mixed Plasmodium falciparum and P. vivax infections undetected by conventional microscopy. Trans R Soc Trop Med Hyg. 1992;86:609–612. - PubMed

[2] Brown A E, Kain K C, Pipithkul J, Webster H K. Demonstration by the polymerase chain reaction of mixed Plasmodium falciparum and P. vivax infections undetected by conventional microscopy. Trans R Soc Trop Med Hyg. 1992;86:609–612. - PubMed

[3] Casey J, Davidson N. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. Nucleic Acids Res. 1977;4:1539–1552. - PMC - PubMed

[4] Casey J, Davidson N. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. Nucleic Acids Res. 1977;4:1539–1552. - PMC - PubMed

[5] Humar A, Harrington M A, Kain K C. Evaluation of a non-isotopic polymerase chain reaction-based assay to detect and predict treatment failure of Plasmodium vivax malaria in travelers. Trans R Soc Trop Med Hyg. 1997;91:406–409. - PubMed

[6] Humar A, Harrington M A, Kain K C. Evaluation of a non-isotopic polymerase chain reaction-based assay to detect and predict treatment failure of Plasmodium vivax malaria in travelers. Trans R Soc Trop Med Hyg. 1997;91:406–409. - PubMed

[7] Humar A, Ohrt C, Harrington M A, Pillai D, Kain K C. ParaSight®F test compared with the polymerase chain reaction and microscopy for the diagnosis of Plasmodium falciparum malaria in travelers. Am J Trop Med Hyg. 1997;55:44–48. - PubMed

[8] Humar A, Ohrt C, Harrington M A, Pillai D, Kain K C. ParaSight®F test compared with the polymerase chain reaction and microscopy for the diagnosis of Plasmodium falciparum malaria in travelers. Am J Trop Med Hyg. 1997;55:44–48. - PubMed

[9] Kain K C, Brown A E, Mirabelli L, Webster H K. Detection of Plasmodium vivax by polymerase chain reaction in a field study. J Infect Dis. 1993;168:1323–1326. - PubMed

[10] Kain K C, Brown A E, Mirabelli L, Webster H K. Detection of Plasmodium vivax by polymerase chain reaction in a field study. J Infect Dis. 1993;168:1323–1326. - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Evaluation of a colorimetric PCR-based assay to diagnose Plasmodium falciparum malaria in travelers

Affiliation

Evaluation of a colorimetric PCR-based assay to diagnose Plasmodium falciparum malaria in travelers

Authors

Affiliation

Abstract

Similar articles

Cited by

References

Publication types

MeSH terms

LinkOut - more resources

Full Text Sources

Abstract

Similar articles

Cited by

References

Publication types

MeSH terms

Related information

LinkOut - more resources

Full Text Sources