Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1999 Feb;37(2):367-70.
doi: 10.1128/JCM.37.2.367-370.1999.

Evaluation of a rapid immunochromatographic test for detection of antibodies to human immunodeficiency virus

H Arai  1 , B Petchclai, K Khupulsup, T Kurimura, K Takeda
Affiliations

Evaluation of a rapid immunochromatographic test for detection of antibodies to human immunodeficiency virus

H Arai et al. J Clin Microbiol. 1999 Feb.

Abstract

A new immunochromatographic rapid test, Determine HIV-1/2, for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1) and HIV-2 in human whole blood, serum, and plasma was evaluated. Determine HIV-1/2 is a sandwich immunoassay and uses a nitrocellulose strip with a capture site for the patient's results and a procedural control site to confirm the validity of the assay. The results can be read visually, and a positive result is indicated by the formation of a red line within 15 min after sample application. The test showed 100% sensitivity for HIV-1 with 102 whole-blood, 152 serum, and 144 plasma samples obtained from Ramathibodi Hospital, Bangkok, Thailand. The sensitivity of the test for HIV-2 was 100% with 100 serum or plasma samples obtained from Ivory Coast. The sensitivity of the test with 4 anti-HIV-1 seroconversion panels from Boston Biomedica Inc. was equivalent to or better than those of another agglutination assay with serum or plasma and the enzyme immunoassay licensed by the U.S. Food and Drug Administration. The specificity was 100% with 367 sets of whole-blood, serum, and plasma samples from Ramathibodi Hospital. This method had an analytical sensitivity for the detection of HIV-1 equivalent to or better than that of another agglutination assay with serum or plasma. This test had an analytical sensitivity for the detection of HIV-1 better than that of another immunochromatographic test with whole blood. This evaluation demonstrated the excellent performance of this immunochromatographic test with EDTA-anticoagulated whole-blood, serum, and plasma samples. We conclude that this test is suitable for use in emerging countries and is an excellent alternative to HIV antibody testing at remote sites, as well as in traditional laboratories.

PubMed Disclaimer

References

    1. Barre-Sinoussi F, Chermann J C, Rey F, et al. Isolation of T-lymphotropic retrovirus from patient at risk for acquired immunodeficiency syndrome (AIDS) Science. 1983;220:868–971. - PubMed
    1. Behets F, Disasi A, Ryder R W, Bishagara K, Piot P, Kashamuka M, Kamenga M, Nzila N, Laga M, Vercanteren G, Batter V, Brown C, Quinn T. Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa. J Clin Microbiol. 1991;29:2280–2284. - PMC - PubMed
    1. Carson J L, Russell L B, Taragin M I, Sonnenberg F A, Duff A E, Bauer S. The risk of blood transfusion: the relative influence of acquired immunodeficiency syndrome and non-A, non-B hepatitis. Am J Med. 1992;92:45–52. - PubMed
    1. Centers for Disease Control. Update: serologic testing for HIV-1 antibody—United States. Morbid Mortal Weekly Rep. 1990;39:69–72. - PubMed
    1. Centers for Disease Control and Prevention. Testing for antibodies to human immunodeficiency virus type 2 in the United States. Morbid Mortal Weekly Rep. 1992;41:R1–R9. - PubMed

LinkOut - more resources