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Comparative Study
. 1999 Feb;37(2):447-9.
doi: 10.1128/JCM.37.2.447-449.1999.

Comparison of NucliSens and Roche Monitor assays for quantitation of levels of human immunodeficiency virus type 1 RNA in plasma

J R Dyer  1 , C D Pilcher, R Shepard, J Schock, J J Eron, S A Fiscus
Affiliations
Comparative Study

Comparison of NucliSens and Roche Monitor assays for quantitation of levels of human immunodeficiency virus type 1 RNA in plasma

J R Dyer et al. J Clin Microbiol. 1999 Feb.

Abstract

We compared the performance of Organon Teknika's NucliSens and Roche Diagnostic Systems' Monitor quantitative human immunodeficiency type 1 RNA assays. Both had similar linearity and sensitivity over most of the dynamic range of the assays, although the Monitor assay was superior at the low range of RNA values while the NucliSens assay was more consistent at higher RNA values. NucliSens generally showed less interassay variability.

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Figures

FIG. 1
FIG. 1
HIV-1 RNA concentrations in patient samples tested by NucliSens plotted against data obtained with Monitor, before (a) and after (b) adjustment by using VQAL standards. The solid line is the line of unity. (c and d) Difference between log10 Monitor and NucliSens data plotted against the mean of the log10 HIV-1 RNA concentrations measured with the two assays, before (c) and after (d) adjustment.
See this image and copyright information in PMC

References

    1. Brambilla D, Leung S, Lew J, Todd J, Herman S, Cronin M, Shapiro D E, Bremer J, Hanson C, Hillyer G V, Sherry G D M, Sperling R S, Coombs R W, Reichelderfer P S. Absolute copy number and relative change in determinations of human immunodeficiency virus type 1 RNA in plasma: effect of an external standard on kit comparisons. J Clin Microbiol. 1998;36:311–314. - PMC - PubMed
    1. Dyer J R, Pilcher C D, Shepard R, Schock J, Eron J J, Fiscus S A. Abstr. 42162. 12th World AIDS Conference, Geneva, Switzerland. 1998.
    1. Finzi D, Siliciano R F. Viral dynamics in HIV-1 infection. Cell. 1998;93:665–671. - PubMed
    1. Griffith B P, Rigsby M O, Garner R B, Gordon M M, Chacko T M. Comparison of the Amplicor HIV-1 Monitor test and the nucleic acid sequence-based amplification assay for quantitation of human immunodeficiency virus RNA in plasma, serum, and plasma subjected to freeze-thaw cycles. J Clin Microbiol. 1997;35:3288–3291. - PMC - PubMed
    1. Lew J, Reichelderfer P, Fowler M, et al. Determinations of levels of human immunodeficiency virus type 1 RNA in plasma: reassessment of parameters affecting assay outcome. J Clin Microbiol. 1998;36:1471–1479. - PMC - PubMed

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