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. 1999 Feb;37(2):461-3.
doi: 10.1128/JCM.37.2.461-463.1999.

PCR targeted to the 16S-23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes

S L Stubbs  1 , J S Brazier, G L O'Neill, B I Duerden
Affiliations

PCR targeted to the 16S-23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes

S L Stubbs et al. J Clin Microbiol. 1999 Feb.

Abstract

A reference library of types of Clostridium difficile has been constructed by PCR ribotyping isolates (n = 2,030) from environmental (n = 89), hospital (n = 1,386), community practitioner (n = 395), veterinary (n = 27), and reference (n = 133) sources. The library consists of 116 distinct types identified on the basis of differences in profiles generated with PCR primers designed to amplify the 16S-23S rRNA gene intergenic spacer region. Isolates from 55% of infections in hospitals in the United Kingdom belonged to one ribotype (type 1), but this type was responsible for only 7. 5% of community infections.

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Figures

FIG. 1
FIG. 1
PCR ribotype profiles obtained with strains belonging to PCR ribotypes 81 to 90. Lane L refers to 100-bp ladder (200 bp − 1 kbp).
See this image and copyright information in PMC

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