Preparation and storage of platelet concentrates. I. Factors influencing the harvest of viable platelets from whole blood

Abstract

Factors affecting the yield and viability of concentrated platelets have been investigated in a blood component programme. It was found that 86+/-1% of the platelets from a unit of whole blood can be concentrated without loss of viability by processing ACD or CPD anticoagulated blood at room temperature. The steps are initial centrifugation at 1000 g for 9 min to harvest platelet-rich plasma; centrifugation of the platelet-rich plasma at 3000 g for 20 min to pack the platelets; and resuspension of the platelet pellet in residual plasma after 1 1/2 h.