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Review
. 1999 Jan;21(1 Suppl):10-4.
doi: 10.1038/4434.

Expression profiling using cDNA microarrays

D J Duggan  1 , M Bittner, Y Chen, P Meltzer, J M Trent
Affiliations
Review

Expression profiling using cDNA microarrays

D J Duggan et al. Nat Genet. 1999 Jan.

Abstract

cDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. DNA targets, in the form of 3' expressed sequence tags (ESTs), are arrayed onto glass slides (or membranes) and probed with fluorescent- or radioactively-labelled cDNAs. Here, we review technical aspects of cDNA microarrays, including the general principles, fabrication of the arrays, target labelling, image analysis and data extraction, management and mining.

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