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. 1998;45(3):669-76.

Cloning and sequencing of cDNA encoding the rice methionyl-tRNA synthetase

M Deniziak  1 , M Mirande, J Barciszewski
Affiliations
  • PMID: 9918493

Cloning and sequencing of cDNA encoding the rice methionyl-tRNA synthetase

M Deniziak et al. Acta Biochim Pol. 1998.

Abstract

Three overlapping clones of cDNA, Mos43, Mos28 and Mos60, coding for methionyl-tRNA synthetase were obtained by screening the Oryza sativa lambda gt11 library. Their nucleotide sequence of 2850 bp was determined. The deduced amino-acid sequence of the isolated clones contains a HLGN and KFSKS motifs, which are conserved for this family of enzymes and have been proposed to be the signature sequences for class I aminoacyl-tRNA synthetases. A comparison of the rice MetRS primary structure with those deposited in EMBL/GenBank points to its high homology to yeast, human and Caenorhabditis elegans MetRSs. Interestingly, a great similarity of its C terminus to endothelial-monocyte-activating polypeptide II (EMAPII) and yeast protein G4p1 was observed.

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