doi: 10.1016/s0014-5793(98)01635-4.
Recombinant human amyloid precursor-like protein 2 (APLP2) expressed in the yeast Pichia pastoris can stimulate neurite outgrowth
Affiliations
- PMID: 9923612
- DOI: 10.1016/s0014-5793(98)01635-4
Item in Clipboard
Recombinant human amyloid precursor-like protein 2 (APLP2) expressed in the yeast Pichia pastoris can stimulate neurite outgrowth
FEBS Lett.
.
Free article
Abstract
The human amyloid precursor-like protein 2 (APLP2) is a member of the Alzheimer's disease amyloid precursor protein (APP) gene family. The human APLP2 ectodomain (sAPLP2) was expressed in the yeast Pichia pastoris and the recombinant sAPLP2 was purified from the culture medium in a single step by metal-chelating Sepharose chromatography. The neuritotrophic activity of APLP2 was compared to the APP isoforms sAPP695 and sAPP751 on chick sympathetic neurones. APLP2 had neurite outgrowth-promoting activity similar to that of the APP isoforms. This suggests that APP and APLP2 have a similar or related role and supports the idea of a redundancy in function between the APP-gene family proteins.
Similar articles
-
A heparin-binding domain in the amyloid protein precursor of Alzheimer's disease is involved in the regulation of neurite outgrowth.J Neurosci. 1994 Apr;14(4):2117-27. doi: 10.1523/JNEUROSCI.14-04-02117.1994. J Neurosci. 1994. PMID: 8158260 Free PMC article.
-
Accumulation of the amyloid precursor-like protein APLP2 and reduction of APLP1 in retinoic acid-differentiated human neuroblastoma cells upon curcumin-induced neurite retraction.Brain Res Mol Brain Res. 2003 Nov 6;119(1):62-72. doi: 10.1016/j.molbrainres.2003.08.014. Brain Res Mol Brain Res. 2003. PMID: 14597230
-
Copper levels are increased in the cerebral cortex and liver of APP and APLP2 knockout mice.Brain Res. 1999 Sep 25;842(2):439-44. doi: 10.1016/s0006-8993(99)01861-2. Brain Res. 1999. PMID: 10526140
-
Amyloid precursor protein and amyloid precursor-like protein 2 in cancer.Oncotarget. 2016 Apr 12;7(15):19430-44. doi: 10.18632/oncotarget.7103. Oncotarget. 2016. PMID: 26840089 Free PMC article. Review.
-
Redundancy and divergence in the amyloid precursor protein family.FEBS Lett. 2013 Jun 27;587(13):2036-45. doi: 10.1016/j.febslet.2013.05.026. Epub 2013 May 23. FEBS Lett. 2013. PMID: 23707420 Review.
Cited by
-
Recombinant protein expression in Pichia pastoris.Mol Biotechnol. 2000 Sep;16(1):23-52. doi: 10.1385/MB:16:1:23. Mol Biotechnol. 2000. PMID: 11098467 Review.
-
Modulation of BAG3 Expression and Proteasomal Activity by sAPPα Does Not Require Membrane-Tethered Holo-APP.Mol Neurobiol. 2016 Nov;53(9):5985-5994. doi: 10.1007/s12035-015-9501-y. Epub 2015 Nov 2. Mol Neurobiol. 2016. PMID: 26526841
-
Amyloid Precursor-like Protein 2 Expression Increases during Pancreatic Cancer Development and Shortens the Survival of a Spontaneous Mouse Model of Pancreatic Cancer.Cancers (Basel). 2021 Mar 26;13(7):1535. doi: 10.3390/cancers13071535. Cancers (Basel). 2021. PMID: 33810510 Free PMC article.
-
Solution studies and structural model of the extracellular domain of the human amyloid precursor protein.Biophys J. 2002 Dec;83(6):3513-24. doi: 10.1016/S0006-3495(02)75351-4. Biophys J. 2002. PMID: 12496118 Free PMC article.
-
Amyloid precursor-like protein 2 increases the endocytosis, instability, and turnover of the H2-K(d) MHC class I molecule.J Immunol. 2008 Aug 1;181(3):1978-87. doi: 10.4049/jimmunol.181.3.1978. J Immunol. 2008. PMID: 18641335 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
