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. 1999 Feb;43(2):377-80.
doi: 10.1128/AAC.43.2.377.

Human herpesvirus 8 DNA load in leukocytes of human immunodeficiency virus-infected subjects: correlation with the presence of Kaposi's sarcoma and response to anticytomegalovirus therapy

G Boivin  1 , A Gaudreau, E Toma, R Lalonde, J P Routy, G Murray, J Handfield, M G Bergeron
Affiliations

Human herpesvirus 8 DNA load in leukocytes of human immunodeficiency virus-infected subjects: correlation with the presence of Kaposi's sarcoma and response to anticytomegalovirus therapy

G Boivin et al. Antimicrob Agents Chemother. 1999 Feb.

Abstract

Specific human herpesvirus 8 (HHV-8) DNA sequences were found in leukocytes of 12 of 29 (41.4%) AIDS subjects with Kaposi's sarcoma (KS), whereas they were found in 4 of 43 (9.3%) AIDS subjects without KS (P = 0.003), although the peak HHV-8 DNA load in PCR-positive subjects with KS (mean, 425 copies per 0.2 microgram of DNA) did not significantly differ from the one found in PCR-positive patients without KS (mean, 218 copies). The use of intravenous ganciclovir or foscarnet therapy to treat cytomegalovirus disease did not affect the HHV-8 DNA load in seven patients for whom serial samples were analyzed.

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Figures

FIG. 1
FIG. 1
Quantitative-competitive PCR for HHV-8. Differential hybridization of PCR products was done with an internal control probe (A) and an HHV-8-specific probe (B). Lane 1, blank; lane 2, background of HHV-8 DNA-negative leukocytes; lane 3, 100 copies of the internal control in a background of HHV-8 DNA-negative leukocytes; lanes 4 to 11, 100 copies of the internal control coamplified with increasing concentrations of the HHV-8 external standard (25, 50, 100, 500, 1,000, 5,000, 10,000, and 15,000 copies, respectively) in a background of HHV-8 DNA-negative leukocytes.
See this image and copyright information in PMC

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