Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1999 Feb;43(2):397-9.
doi: 10.1128/AAC.43.2.397.

Resistance of Pseudomonas aeruginosa to imipenem induced by eluates from siliconized latex urinary catheters is related to outer membrane protein alterations

L Martínez-Martínez  1 , A Pascual, M C Conejo, L Picabea, E J Perea
Affiliations

Resistance of Pseudomonas aeruginosa to imipenem induced by eluates from siliconized latex urinary catheters is related to outer membrane protein alterations

L Martínez-Martínez et al. Antimicrob Agents Chemother. 1999 Feb.

Abstract

The activity of imipenem against Pseudomonas aeruginosa HUS-3 decreased by 16 times in the presence of substances eluted from siliconized latex urinary catheters (SLUCs). SLUCs did not inactivate imipenem or increase beta-lactamase activity. The outer membrane of P. aeruginosa HUS-3 grown in the presence of eluate lacked an OprD-like protein and expressed a new 50-kDa protein. The decreased activity of imipenem against P. aeruginosa in the presence of SLUCs is related to the loss of an OprD-like protein and the expression of a new outer membrane protein.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Outer membrane proteins of P. aeruginosa grown in MHB (lanes 1, 3, 5, and 6) or in eluate (lanes 2 and 4). Lanes: 1 and 2, strain HUS-3; 3 and 4, mutant MUT2; 5, strain PAO1; 6, OprD-deficient mutant of PAO1. Molecular markers are shown at right. The ca. 50-kDa new protein expressed in eluate is marked with arrowheads.
See this image and copyright information in PMC

References

    1. Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principal of protein-dye binding. Anal Biochem. 1976;72:248–254. - PubMed
    1. Dinh T, Paulsen I T, Saier M H., Jr A family of extracytoplasmic proteins that allow transport of large molecules across the outer membrane of gram-negative bacteria. J Bacteriol. 1994;176:3825–3831. - PMC - PubMed
    1. Fukuda H, Hosaka M, Hirai K, Iyobe S. New norfloxacin resistance in Pseudomonas aeruginosa PAO. Antimicrob Agents Chemother. 1990;34:1757–1761. - PMC - PubMed
    1. Köhler T, Michéa-Hamzehpour M, Henze U, Gotoh N, Curty L K, Pechère J-C. Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa. Mol Microbiol. 1997;23:343–354. - PubMed
    1. Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 1970;227:680–685. - PubMed

MeSH terms

LinkOut - more resources