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. 1976 Aug;12(8):554-7.
doi: 10.1007/BF02797438.

Preparation of delipidized serum protein for use in cell culture systems

Preparation of delipidized serum protein for use in cell culture systems

G H Rothblat et al. In Vitro. 1976 Aug.

Abstract

A rapid procedure for the preparation of delipidized serum protein is described. The delipidized protein can be used for the maintenance and growth of tissue culture cells in a lipid-free environment. The extraction procedure greatly reduces all serum lipid classes and the delipidized protein supports the growth of a variety of cells in culture.

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References

    1. J Biol Chem. 1957 May;226(1):497-509 - PubMed
    1. Arch Biochem Biophys. 1958 Apr;74(2):390-7 - PubMed
    1. J Lipid Res. 1975 Mar;16(2):151-4 - PubMed
    1. Nature. 1968 Jul 20;219(5151):300 - PubMed
    1. Clin Chim Acta. 1973 Jun 14;46(1):63-8 - PubMed

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