doi: 10.1128/jb.135.3.775-781.1978.
In vivo titration of araC protein
- PMID: 99435
- PMCID: PMC222447
- DOI: 10.1128/jb.135.3.775-781.1978
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In vivo titration of araC protein
J Bacteriol.
1978 Sep.
Abstract
The requirement for araC protein in the induction of the araBAD operon was investigated. Strains of Escherichia coli carrying an araC(Am) mutation and temperature-sensitive amber suppressors were used to vary the intracellular level of araC protein. The levels of araC protein studied ranged from 0.007 to 1.8 times the normal amount. The results indicate that the normal level of araC protein is just sufficient to provide maximal expression of the araBAD operon.
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