pUCPCR1. A vector for direct cloning of PCR products in a double Xcm1 restriction site offering compatible single 3'-overhanging T residues

Abstract

The multiple cloning site of pUC19 was replaced by a multiple cloning site possessing a double Xcm1 restriction site. Digestion with XcmI gives a linear vector with a single 3'-overhanging T-residue at both ends. This provides the easiest way of creating a vector in which PCR fragments produced by Taq polymerase can be directly cloned without further modifications.