Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Dec;10(3):273-4.
doi: 10.1007/BF02740849.

pUCPCR1. A vector for direct cloning of PCR products in a double Xcm1 restriction site offering compatible single 3'-overhanging T residues

E de Vries  1
Affiliations

pUCPCR1. A vector for direct cloning of PCR products in a double Xcm1 restriction site offering compatible single 3'-overhanging T residues

E de Vries. Mol Biotechnol. 1998 Dec.

Abstract

The multiple cloning site of pUC19 was replaced by a multiple cloning site possessing a double Xcm1 restriction site. Digestion with XcmI gives a linear vector with a single 3'-overhanging T-residue at both ends. This provides the easiest way of creating a vector in which PCR fragments produced by Taq polymerase can be directly cloned without further modifications.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1991 Mar 11;19(5):1154 - PubMed
    1. Nucleic Acids Res. 1988 Oct 25;16(20):9677-86 - PubMed
    1. Gene. 1985;33(1):103-19 - PubMed

MeSH terms