Hybrid frequencies confirm limit to coinfection in the RNA bacteriophage phi6

Abstract

Coinfection of the same host cell by multiple viruses may lead to increased competition for limited cellular resources, thus reducing the fitness of an individual virus. Selection should favor viruses that can limit or prevent coinfection, and it is not surprising that many viruses have evolved mechanisms to do so. Here we explore whether coinfection is limited in the RNA bacteriophage phi6 that infects Pseudomonas phaseolicola. We estimated the limit to coinfection in phi6 by comparing the frequency of hybrids produced by two marked phage strains to that predicted by a mathematical model based on differing limits to coinfection. Our results provide an alternative method for estimating the limit to coinfection and confirm a previous estimate between two to three phages per host cell. In addition, our data reveal that the rate of coinfection at low phage densities may exceed that expected through random Poisson sampling. We discuss whether phage phi6 has evolved an optimal limit that balances the costly and beneficial fitness effects associated with multiple infections.

FIG. 1

Comparison between expected and observed values of H, frequency of (+++) hybrids generated in crosses between genotypes MX (+ X +) and LX (++ X). Curves were generated by a mathematical model that predicts H over a range of multiplicities of infection (m), assuming a defined limit to coinfection (N). Expected values for H are adjusted if marked segments experience a replicative disadvantage relative to wild type (see text for details). Observed values of H were generated in two replicate experimental crosses between phages MX and LX at eight multiplicities of infection (0.02, 1, 2, 3, 4, 5, 10, 25). Each point represents an independent estimate. Observed H saturates at a value corresponding to a limit of between two and three phages per cell.