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. 1999 Mar;37(3):581-90.
doi: 10.1128/JCM.37.3.581-590.1999.

Genetic diversity and population structure of Vibrio cholerae

P Beltrán  1 , G Delgado, A Navarro, F Trujillo, R K Selander, A Cravioto
Affiliations

Genetic diversity and population structure of Vibrio cholerae

P Beltrán et al. J Clin Microbiol. 1999 Mar.

Abstract

Multilocus enzyme electrophoresis (MLEE) of 397 Vibrio cholerae isolates, including 143 serogroup reference strains and 244 strains from Mexico and Guatemala, identified 279 electrophoretic types (ETs) distributed in two major divisions (I and II). Linkage disequilibrium was demonstrated in both divisions and in subdivision Ic of division I but not in subdivision Ia, which includes 76% of the ETs. Despite this evidence of relatively frequent recombination, clonal lineages may persist for periods of time measured in at least decades. In addition to the pandemic clones of serogroups O1 and O139, which form a tight cluster of four ETs in subdivision Ia, MLEE analysis identified numerous apparent clonal lineages of non-O1 strains with intercontinental distributions. A clone of serogroup O37 that demonstrated epidemic potential in the 1960s is closely related to the pandemic O1/O139 clones, but the nontoxigenic O1 Inaba El Tor reference strain is not. A strain of serogroup O22, which has been identified as the most likely donor of exogenous rfb region DNA to the O1 progenitor of the O139 clone, is distantly related to the O1/O139 clones. The close evolutionary relationships of the O1, O139, and O37 epidemic clones indicates that new cholera clones are likely to arise by the modification of a lineage that is already epidemic or is closely related to such a clone.

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Figures

FIG. 1
FIG. 1
Dendrogram showing genetic relationships of the ETs of V. cholerae, based on MLEE analysis (17 loci). The dendrogram was constructed from a matrix of pairwise genetic distances by the UPGMA method. The lineages of subdivision Ic and of groups A, B, and C of subdivision Ia are truncated. The relationships of the 37 ETs in group A are shown in the dendrogram in Fig. 2.
FIG. 2
FIG. 2
Dendrogram showing genetic relationships of the 37 ETs of V. cholerae in group A of subdivision Ia of division I, based on MLEE analysis (17 loci). The dendrogram was constructed from a matrix of pairwise genetic distances by the UPGMA method.
See this image and copyright information in PMC

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