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. 1999 Mar;37(3):600-5.
doi: 10.1128/JCM.37.3.600-605.1999.

Typing of clinical Mycobacterium avium complex strains cultured during a 2-year period in Denmark by using IS1245

J Bauer  1 , A B Andersen, D Askgaard, S B Giese, B Larsen
Affiliations

Typing of clinical Mycobacterium avium complex strains cultured during a 2-year period in Denmark by using IS1245

J Bauer et al. J Clin Microbiol. 1999 Mar.

Abstract

In the present study restriction fragment length polymorphism analyses with the recently described insertion sequence IS1245 as a probe was performed with clinical Mycobacterium avium complex strains cultured in Denmark during a 2-year period. The overall aim of the study was to disclose potential routes of transmission of these microorganisms. As a first step, the genetic diversity among isolates from AIDS patients and non-human immunodeficiency virus (HIV)-infected patients was described. In addition, a number of isolates from nonhuman sources cultured during the same period were analyzed and compared to the human isolates. A total of 203 isolates from AIDS patients (n = 90), non-HIV-infected patients (n = 91), and nonhuman sources (n = 22) were analyzed. The presence of IS1245 was restricted to Mycobacterium avium subsp. avium isolates. The majority of human isolates had large numbers of IS1245 copies, while nonhuman isolates could be divided into a high-copy-number group and a low-copy-number group. Groups of identical strains were found to be geographically widespread, comprising strains from AIDS patients as well as strains from non-HIV-infected patients. Samples of peat (to be used as potting soil) and veterinary samples were found to contain viable M. avium isolates belonging to genotypes also found in humans.

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Figures

FIG. 1
FIG. 1
Numbers of bands for M. avium isolates cultured from AIDS patients (□), non-HIV-infected patients (■), and nonhuman sources (▧).
FIG. 2
FIG. 2
Geographical distribution of 23 M. avium clusters. The numbers indicate cluster numbers.
FIG. 3
FIG. 3
Examples of the four most frequent IS1245 patterns found among 179 human and nonhuman M. avium isolates.
See this image and copyright information in PMC

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