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Comparative Study
. 1999 Mar;37(3):721-3.
doi: 10.1128/JCM.37.3.721-723.1999.

Sensitivity and specificity of dipstick tests for rapid diagnosis of malaria in nonimmune travelers

T Jelinek  1 , M P Grobusch, S Schwenke, S Steidl, F von Sonnenburg, H D Nothdurft, E Klein, T Löscher
Affiliations
Comparative Study

Sensitivity and specificity of dipstick tests for rapid diagnosis of malaria in nonimmune travelers

T Jelinek et al. J Clin Microbiol. 1999 Mar.

Abstract

Swift diagnosis of Plasmodium falciparum malaria in areas where the disease is not endemic is frequently complicated by the lack of experience on the side of involved laboratory personal. Diagnostic tools based on the dipstick principle for the detection of plasmodial histidine-rich protein 2 (HRP-2) and parasite-specific lactate dehydrogenase (pLDH), respectively, have become available for the qualitative detection of P. falciparum malaria. In order to evaluate two of the currently available assays, specimens from 231 patients were screened during a prospective multicenter study. Among the screened specimens, samples from 53 patients (22.9%) were positive for P. falciparum malaria by microscopy and/or PCR. While the test kit based on the detection of HRP-2 performed with a sensitivity of 92.5% and a specificity of 98.3%, the kit for the detection of pLDH showed a sensitivity of 88.5% and a specificity of 99.4%. Dipstick tests have the potential of enhancing speed and accuracy of the diagnosis of P. falciparum malaria, especially if nonspecialized laboratories are involved.

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References

    1. Beadle C, Long G, Weiss W, McElroy P, Maret S, Oloo A, Hoffman S. Diagnosis of malaria by detection of Plasmodium falciparum HRP-2 antigen with a rapid dipstick antigen-capture assay. Lancet. 1994;343:564–568. - PubMed
    1. Di Perri G, Olliaro P, Nardi S, Allegranzi B, Deganello R, Vento S, Lanzafame M, Cazzadori A, Bonora S, Concia E. The ParaSight-F rapid dipstick antigen capture assay for monitoring parasite clearance after drug treatment of Plasmodium falciparum malaria. Trans R Soc Trop Med Hyg. 1997;91:403–405. - PubMed
    1. Garcia M, Kirimoama S, Marlborough D, Leafasia J, Rieckmann K. Immunochromatographic test for malaria diagnosis. Lancet. 1996;347:1549. - PubMed
    1. Humar A, Ohrt C, Harrington M, Pillai D, Kain K. ParaSight-F test compared with the polymerase chain reaction and microscopy for the diagnosis of Plasmodium falciparum malaria in travelers. Am J Trop Med Hyg. 1997;56:44–48. - PubMed
    1. Jelinek T, Pröll S, Hess F, von Sonnenburg F, Kabagambe K, Löscher T, Kilian A H D. Geographical differences in the sensitivity of polymerase chain reaction for detection of P. falciparum infection in Uganda. Am J Trop Med Hyg. 1996;55:647–651. - PubMed

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