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Comparative Study
. 1999 Mar;37(3):772-4.
doi: 10.1128/JCM.37.3.772-774.1999.

Comparison of five PCR methods for detection of Helicobacter pylori DNA in gastric tissues

Affiliations
Comparative Study

Comparison of five PCR methods for detection of Helicobacter pylori DNA in gastric tissues

J J Lu et al. J Clin Microbiol. 1999 Mar.

Abstract

Five different PCR methods for the detection of Helicobacter pylori were evaluated. The results of this study indicate that of the five PCR methods examined, the ureC (glmM) gene PCR is the most sensitive and specific for the detection of H. pylori in gastric biopsy specimens.

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References

    1. Bickley J, Owen R J, Fraser A G, Pounder R E. Evaluation of the polymerase chain reaction for detecting the urease C gene of Helicobacter pylori in gastric biopsy samples and dental plaque. J Med Microbiol. 1993;39:338–344. - PubMed
    1. Chong S K F, Lou Q, Fitzgerald J F, Lee C H. Evaluation of the 16S rRNA gene PCR with primers Hp1 and Hp2 for detection of Helicobacter pylori. J Clin Microbiol. 1996;34:2728–2730. - PMC - PubMed
    1. Clayton C L, Kleanthous H, Coates P J, Morgan D D, Tabaqchali S. Sensitive detection of Helicobacter pylori by using polymerase chain reaction. J Clin Microbiol. 1992;30:192–200. - PMC - PubMed
    1. De Reuse H, Labigne A, Mengin-Lecreulx D. The Helicobacter pylori ureC gene codes for a phosphoglucosamine mutase. J Bacteriol. 1997;179:3488–3493. - PMC - PubMed
    1. Foxall P A, Hu L T, Mobley H L. Use of polymerase chain reaction-amplified Helicobacter pylori urease structural genes for differentiation of isolates. J Clin Microbiol. 1992;30:739–741. - PMC - PubMed

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